a crisprcas9 toolkit for multiplex genome editing in plants

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

We developed a toolkit that facilitates transient or stable expression of the CRISPR/Cas9 system in a variety of plant species, which will facilitate plant research, as it enables high efficiency generation of mutants bearing multiple gene mutations. A CRISPR/Cas9 toolkit for multiplex genome editing in plants BMC Plant Biol. 2014 Nov 29;14:327. doi: 10.1186/s12870-014-0327-y. Authors Hui-Li ...

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

29/11/2014  We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides Bsa I to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

A CRISPR/Cas9 toolkit for multiplex genome editing in plants Hui-Li Xing†, Li Dong†, Zhi-Ping Wang, Hai-Yan Zhang, Chun-Yan Han, Bing Liu, Xue-Chen Wang and Qi

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants.

29/11/2014  We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

The CRISPR/Cas9 system is a widely used tool for genome editing in plants. In Arabidopsis (Arabidopsis thaliana), egg cell-specific promoters driving Cas9 expression have been applied to reduce ...

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

BackgroundTo accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/ CRISPR-associated protein 9) system to a variety of plant species, a toolkit with additional plant selectable markers, more gRNA modules, and easier methods for the assembly of one or more gRNA expression cassettes is required.ResultsWe developed a CRISPR/Cas9 binary vector ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Results: We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Results We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Results: We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

BackgroundTo accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/ CRISPR-associated protein 9) system to a variety of plant species, a toolkit with additional plant selectable markers, more gRNA modules, and easier methods for the assembly of one or more gRNA expression cassettes is required.ResultsWe developed a CRISPR/Cas9 binary vector ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

A CRISPR/Cas9 toolkit for multiplex genome editing in plants Author: Xing, Hui-Li Dong, Li Wang, Zhi-Ping Zhang, Hai-Yan Han, Chun-Yan Liu, Bing Wang, Xue-Chen Chen, Qi-Jun Journal: BMC Plant Biology Issue Date: 2014 Abstract(summary): Background: To accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/ CRISPR-associated protein

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants.

We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step. The ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants.

RESULTS: We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

A CRISPR/Cas9 toolkit for multiplex genome editing in plants BMC Plant Biology , Nov 2014 Hui-Li Xing , Li Dong , Zhi-Ping Wang , Hai-Yan Zhang , Chun-Yan

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants.

Background: To accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of plant species, a toolkit with additional plant selectable markers, more gRNA modules, and easier methods for the assembly of one or more gRNA expression cassettes is required.

Get Price

Protocol : A CRISPR/Cas9 toolkit for multiplex genome ...

We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step. The ...

Get Price

CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing ...

While we were preparing our article, Xing et al. (2014) and Ma et al. (2015) reported a CRISPR/Cas9 toolkit for targeted mutagenesis in plants. They showed simultaneous knockout of multiple genes in Arabidopsis and rice, which, again, demonstrates the versatility and power of a multiplex CRISPR/Cas9 genome-editing system. Compared with their ...

Get Price

Multipurpose Toolkit to Enable Advanced Genome Engineering ...

Genome engineering promises to advance both basic and applied plant research, and we developed a comprehensive reagent toolkit to make the latest genome engineering technologies available to the plant science community. Both TALENs and CRISPR/Cas9 can be easily combined with a variety of regulatory sequences, transformation vectors, and other DNA modifying enzymes, making it possible

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Results: We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Academic research paper on topic "A CRISPR/Cas9 toolkit for multiplex genome editing in plants" Plant Biology . METHODOLOGY ARTICLE Open Access. A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Hui-Li Xing+, Li Dong+, Zhi-Ping Wang, Hai-Yan Zhang, Chun-Yan Han, Bing Liu, Xue-Chen Wang and Qi-Jun Chen* Abstract. Background: To accelerate the application of the CRISPR/Cas9 ...

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

A CRISPR/Cas9 toolkit for multiplex genome editing in plants BMC Plant Biology , Nov 2014 Hui-Li Xing , Li Dong , Zhi-Ping Wang , Hai-Yan Zhang , Chun-Yan

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants.

Background: To accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of plant species, a toolkit with additional plant selectable markers, more gRNA modules, and easier methods for the assembly of one or more gRNA expression cassettes is required.

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Genome-Wide Prediction of Highly Specific Guide RNA Spacers for CRISPR–Cas9-Mediated Genome Editing in Model Plants and Major Crops (2014) Kabin Xie et al. Molecular Plant

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

A CRISPR/Cas9 toolkit for multiplex genome editing in plants By Hui-Li Xing, Li Dong, Zhi-Ping Wang, Hai-Yan Zhang, Chun-Yan Han, Bing Liu, Xue-Chen Wang and Qi-Jun Chen Cite

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Protocol : A CRISPR/Cas9 toolkit for multiplex genome ...

We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step. The ...

Get Price

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

RESULTS We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one

Get Price

CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing ...

While we were preparing our article, Xing et al. (2014) and Ma et al. (2015) reported a CRISPR/Cas9 toolkit for targeted mutagenesis in plants. They showed simultaneous knockout of multiple genes in Arabidopsis and rice, which, again, demonstrates the versatility and power of a multiplex CRISPR/Cas9 genome-editing system. Compared with their ...

Get Price

Multipurpose Toolkit to Enable Advanced Genome Engineering ...

Genome engineering promises to advance both basic and applied plant research, and we developed a comprehensive reagent toolkit to make the latest genome engineering technologies available to the plant science community. Both TALENs and CRISPR/Cas9 can be easily combined with a variety of regulatory sequences, transformation vectors, and other DNA modifying enzymes, making it possible

Get Price